What is the difference between the growth of Gram-positive cocci and gram-negative bacteria on their pattern: 1 TSA or SBS2. EMB or MAC3. Enterococci media (under the assumption that some organisms Neisseria / spirochetes and urine cultures)
Difference between Gram-positive cocci and gram-negative?
Difference between Gram-positive cocci and gram-negative?
You can follow all the replies to this entry through the comments feed.
TSA is an Undefined media and will grow a large number of things. This is usually used for an overall evaluation of quantity present, not for differentiation.
SBS is Brilliant Green Agar with Sulfapyridine. This is a differential media that will only grow Salmonella spp. (Gram negative rods)
EMB is Eosin Methylene Blue Agar is used to grown coliforms (Gram negatives). In this media a pH indicator shows the reduction of peptone, helping to distinguish the different coliforms.
MAC is MacConkey’s Agar. This media will only grow Gram negative bacteria. Also included is a pH indicator allowing for the differentiation of S. areus.
Enterococcal media is used to only grow Gram positive cocci.
You can match up the organisms if you know a couple things about them:
Nisseria spp. are Gram negative
Spirochetes and Gram negative